| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 111 | Ezakiella coagulans | AATGGCTGCAGGCGTTGA | AGCACACGACCTGTTGCA | 60.28 | 59.81 | 183 | 110 |
100.00%
|
100.00%
|
| Primer 112 | Ezakiella coagulans | GCACTTGCAGGATGGCTTG | TAACGCCTGCGAGCAAGT | 59.79 | 59.66 | 169 | 110 |
100.00%
|
100.00%
|
| Primer 113 | Ezakiella coagulans | GGCGATGGATGATTTAGCCGA | CCCTTTGATTCCGGAGTAAGC | 60.61 | 58.71 | 219 | 109 |
100.00%
|
100.00%
|
| Primer 114 | Ezakiella coagulans | GCGATGGATGATTTAGCCGA | CCCTTTGATTCCGGAGTAAGC | 58.20 | 58.71 | 218 | 109 |
100.00%
|
100.00%
|
| Primer 115 | Ezakiella coagulans | CCAAGCTCCGACTCCGTTT | TCAACCGCAAGTGCCCTT | 60.00 | 59.81 | 222 | 109 |
100.00%
|
100.00%
|
| Primer 116 | Ezakiella coagulans | GCACGAAGTCGCTAACGGA | GCGAGCGGTGACAAGATTT | 60.44 | 58.84 | 278 | 109 |
100.00%
|
100.00%
|
| Primer 117 | Ezakiella coagulans | TGCCTGGTTGCGGAAAGA | TGCATGCCCTCTCAACGA | 59.49 | 58.93 | 234 | 109 |
100.00%
|
100.00%
|
| Primer 118 | Ezakiella coagulans | GGAGATCCGCTTCGTGCAA | ACGCTCCCATCGCAATGA | 60.45 | 59.42 | 271 | 108 |
100.00%
|
100.00%
|
| Primer 119 | Ezakiella coagulans | ACGTTGCAAAATCGACCGG | TCCGGTCAAAGGAAGGAGC | 59.43 | 59.32 | 191 | 108 |
100.00%
|
100.00%
|
| Primer 120 | Ezakiella coagulans | TCTGCAGGCTTAACACCGG | CCGTGCCAATTGCCCAAA | 60.00 | 59.26 | 172 | 108 |
100.00%
|
100.00%
|